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Separation of a buffy coat sample using the StraightFrom®
Buffy Coat CD3 MicroBead Kit and the MultiMACS™ Cell24 Separator Plus with the Single-Column Adapter and Whole Blood Columns. Cells were fluorescently stained with REAfinity™ Antibodies CD3-FITC, CD14-VioGreen™, as well as with CD45-VioBlue®
and analyzed by flow cytometry using the MACSQuant®
Analyzer. Viable leukocytes were gated for analysis based on scatter signals, propidium iodide fluorescence, and CD45 expression.
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