StraightFrom® Buffy Coat CD14 MicroBead Kit, human

Buffy Coat CD14 MicroBead Kit
, human

The StraightFrom
Buffy Coat CD14 MicroBead Kit has been developed for the rapid positive selection of CD14
cells directly from one buffy coat using the MultiMACS™ Cell24 Separator Plus for semi-automated separation or a MACS
Separator for manual separation. No sample preparation is required, including density gradient centrifugation, erythrocyte lysis, wash steps, or cell count. Whole Blood Columns and Whole Blood Column Elution Buffer are included in the kit to allow direct processing of an entire buffy coat.

Background information

The CD14 antigen belongs to the LPS receptor complex. It is strongly expressed on most monocytes and macrophages and weakly on neutrophils.

Detailed separation procedure

One entire buffy coat is labeled with StraightFrom
Buffy Coat CD14 MicroBeads and loaded onto Whole Blood Columns, which are placed in the magnetic field of a MultiMACS™ Cell24 Separator Plus or a MACS
Separator. After removing the column from the magnetic field, the magnetically retained CD14
cells can be eluted as the positively selected cell fraction.

Downstream applications

The purified CD14
cells are well-suited for further flow cytometric, functional, or molecular analysis.

How to isolate immune cells directly from blood

Follow this fast and easy protocol for magnetic isolation of immune cells directly from blood or blood products without the need for density gradient centrifugation.

Product options: 1
for 1
buffy coat
CHF  739.00

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