StraightFrom® LRSC MicroBeads allow for magnetic cell separation directly from Leukoreduction System Chambers (LRSCs). Leukocyte subsets such as CD4+, CD8+, CD14+, CD19+, or CD56+ cells can be purified using using either MultiMACS™ Instruments or manual separators. Pre-enrichment of white blood cells, e.g. by density gradient centrifugation, is not required. The purified cells are well-suited for further flow cytometric analyses, molecular biology applications, and functional studies.
Separation of a LRSC sample using the StraightFrom® LRSC CD8 MicroBead Kit, human and the MultiMACS™ Cell24 Separator Plus with the Single-Column Adapter and LS Columns. Cells were fluorescently stained with CD3-APC, CD8-PE, as well as CD45-VioBlue® and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
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