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Mouse spleen was dissociated using the gentleMACS™ Dissociator. Sca-1 expressing cells were isolated using Anti-Sca-1 MicroBeads, an MS Column, and a QuadroMACS™ Separator. Cells were fluorescently stained with CD45-APC (# 130-102-783) and Anti-Sca-1-FITC (# 130-102-831) and analyzed using the MACSQuant®
cells after enrichment
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