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A tumor induced by the B16F10 cell line was dissociated using the gentleMACS™ Octo Dissociator with Heaters in combination with the Tumor Dissociation Kit, mouse. CD45+
TILs were isolated from the resulting single-cell suspension using CD45 (TIL) MicroBeads, mouse, an MS Column, and a MiniMACS™ Separator.
Cells were fluorescently stained with CD45-PE and Labeling Check Reagent-VioBlue®
and analyzed by flow cytometry using the MACSQuant®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
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