CD8+ CD45RA+ Effector T Cell Isolation Kit, human

CD8
+
CD45RA
+
Effector T Cell Isolation Kit
, human

The CD8
+
CD45RA
+
Effector T Cell Isolation Kit has been developed for the positive selection of CD8
+
CD45RA
+
effector T cells from human PBMCs.

Background information

The CD8
+
CD45RA
+
Effector T Cell Isolation Kit is ideal for the isolation of cytotoxic T cells that express high levels of perforin and granzyme B
1
. Isolated cells can be analyzed for interactions with other cell types and degranulation or secretion of effector molecules.

Detailed separation procedure

The isolation of CD8
+
CD45RA
+
effector T cells is performed in a two-step procedure. First, non-target cells are indirectly magnetically labeled for depletion with biotin-conjugated antibodies against CD45RO and CD56 and an APC-conjugated antibody against CD197 (CCR7), as well as Anti-APC MicroBeads and Anti-Biotin MicroBeads. In the second step, the CD8
+
CD45RA
+
effector T cells are directly labeled with CD8 MicroBeads and isolated by positive selection from the pre-enriched cell fraction.

Columns

For positive selection: MS, LS, or autoMACS
®
Columns. For depletion: LD or autoMACS Columns.
  • Selected references

    1. Hamann, D. et al. (1997)
      Phenotypic and functional separation of memory and effector human CD8
      +
      T cells.
      J. Exp. Med. 186: 1407-1418
Product options: 1
for 2×
10
9
total cells
CHF  1'133.00

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