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A single-cell suspension from mouse spleen was prepared using the program m_spleen_01.01 on the gentleMACS Dissociator. CD4+
T cells were isolated from this single-cell suspension using the CD4+
T Cell Isolation Kit, an LS Column, and a MidiMACS™ Separator. Cells were fluorescently stained with the MC CD4 T Cell Cocktail (# 130-092-916) and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
T cells after depletion of non-CD4+
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