CD127 MicroBead Kit, human

CD127 MicroBead Kit, human

The CD127 MicroBead Kit has been developed for the positive selection or depletion of CD127
+
cells from human PBMCs.

Background information

CD127, the α-chain of the IL-7 receptor, is a type I membrane glycoprotein. Signaling of IL-7 through the IL-7R requires both IL-7Rα and the common cytokine gamma chain (γc).
1
CD127 can be identified on immature B cells through the early pre-B stage, on thymocytes, and on most mature T cells with transient down-regulation upon activation.
2,3
On regulatory T cells CD127 is absent
4
and its expression is inversely correlated with FoxP3 expression and suppressive function.
5,6
CD127 is also used by thymic stromal derived lymphopoietin (TSLP) as part of a complex.
1
Human regulatory T cells can be isolated, for example, by using the CD4
+
CD25
+
CD127
dim/–
Regulatory T Cell Isolation Kit, human.

Columns

For positive selection: MS, LS, XS, or autoMACS
®
Columns. For depletion: LD, D, or autoMACS Columns.
  • Selected references

    1. Fry, T. J. and Mackall, C. L. (2002) Interleukin-7: from bench to clinic. Blood 99: 3892-3904
    2. Sudo, T. et al. (1993) Expression and function of the interleukin 7 receptor in murine lymphocytes. Proc. Natl. Acad. Sci. U.S.A. 90: 9125-9129
    3. Armitage, R. J. et al. (1991) Expression of receptors for interleukin 4 and interleukin 7 on human T cells. Adv. Exp. Med. Biol. 292: 121-130
    4. Cupedo, T. et al. (2005) Development and activation of regulatory T cells in the human fetus. Eur. J. Immunol. 35: 383-390
    5. Seddiki, N. et al. (2006) Expression of interleukin (IL)-2 and IL-7 receptors discriminates between human regulatory and activated T cells. J. Exp. Med. 203: 1693-1700
    6. Liu, W. et al. (2006)
      CD127 expression inversely correlates with FoxP3 and suppressive function of human CD4
      +
      T reg cells.
      J. Exp. Med. 203: 1701-1711
Product options: 1
for 1×
10
9
total cells
CHF  703.00

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