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cells were isolated from P2 CD-1 mouse brain tissue using the CD140a (PDGFRα) MicroBead Kit, the Neural Tissue Dissociation Kit (P), the gentleMACS Dissociator, a MiniMACS Separator, and an MS Column. Cells were fluorecently stained with Labeling Check Reagent-APC and analyzed by flow cytometry using the MACSQuant®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
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