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Isolation of untouched monocytes from PBMCs by using the Pan Monocyte Isolation Kit and an LS Column. Cells were fluorescently stained with CD14-FITC, CD16-PE, and Anti-Slan (M-DC8)-APC (# 130-093-031) and analyzed using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and PI fluorescence.
PBMCs before separation
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