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Miltenyi Biotec distribution:
As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
Monocytes were isolated from C57BL/6 bone marrow cells by using the Monocyte Isolation Kit (BM), mouse, an LS Column and a QuadroMACS Separator. The cells were fluorescently stained with CD11b-VioBlue, Lin cocktail (CD3ε-PE, CD45R (B220)-PE, Anti-NK1.1-PE, Anti-Ly-6G-PE, CD49b-PE), Anti-Ly-6C-FITC and Anti-Siglec-F-APC and analyzed by flow cytometry using the MACSQuant Analyser. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.