CD303 (BDCA-2) MicroBead Kit, human

CD303 (BDCA-2) MicroBead Kit, human

The CD303 (BDCA-2) MicroBead Kit has been developed for convenient isolation of plasmacytoid dendritic cells (PDCs) from tissues (i.e., bone marrow or lymph nodes). For PDC enrichment from blood, the CD304 MicroBead Kit or the (Diamond) PDC Isolation Kit, are recommended.

Background information

Like CD304 (BDCA-4/Neuropilin-1), the CD303 (BDCA-2) antigen
1-4
is a specific marker for CD11c
CD123
+
plasmacytoid dendritic cells (PDCs) in blood
1,3
and bone marrow
5
. Additionally, CD303 (BDCA-2) expression is strictly confined to PDCs in tissue, e.g., tonsils or lymph nodes
3,5
. CD303 (BDCA-2) is a novel type II transmembrane C-type lectin
3.5
. Unlike binding of antibodies to CD304 (BDCA-4), binding of antibodies to CD303 (BDCA-2) inhibits IFN type I production, which is induced in PDCs by, e.g., influenza virus, CpG oligonucleotides, or serum of systemic lupus erythematosus patients
3,5,7
. Remarkably, PDCs can take up ligand via CD303 (BDCA-2) and process and present ligand to T cells
1,3
. CD303 (BDCA-2)
+
dendritic cells show a plasmacytoid morphology, express the pre-T cell receptor α-chain and are CD4
+
, CD45RA
+
, CD304 (BDCA-4/Neuropilin-1)
+
, CD141 (BDCA-3)
dim
, CD1c (BDCA-1)
, and CD2
. They lack expression of lineage markers (CD3, CD14, CD16, CD19, CD20, CD56) and express neither myeloid markers such as CD13 and CD33, nor Fc receptors such as CD32, CD64, or FcεRI
1
.Unlike CD304 (BDCA-4/Neuropilin-1), CD303 (BDCA-2) is not detectable on CD1a
+
dendritic cells generated
ex vivo
from monocytes or hematopoietic precursor cells.

Downstream applications

PDCs were isolated for functional analyses and compared with other dendritic cell subsets.
1,3
Furthermore, mRNA was isolated from enriched CD303 (BDCA-2)
+
cells for expression cloning of CD303 (BDCA-2)
3
or for analyses on expression of DCAL-1 by RT-PCR
6
. The kit can also be used to deplete CD303 (BDCA-2)
+
cells from, e.g., tonsillar cell suspensions
8
.

Columns

MS, LS, or autoMACS
®
Columns.
  • Selected references

    1. Dzionek, A. et al. (2000) BDCA-2, BDCA-3, BDCA-4: Three markers for distinct subsets of dendritic cells in human peripheral blood. J. Immunol. 165: 6037-6046
    2. Grabbe, S. et al. (2000) Dendritic cells: multi-lineal and multi-functional. Immunol. Today 21: 431-433
    3. Dzionek, A. et al. (2001) BDCA-2, a novel plasmacytoid dendritic cell–specific type II C-type lectin, mediates antigen capture and is a potent inhibitor of interferon α/β induction. J. Exp. Med. 194: 1823-1834
    4. Rönnblom, L. and Alm, G. V. (2001) A pivotal role for the natural interferon α-producing cells (plasmacytoid dendritic cells) in the pathogenesis of lupus. J. Exp. Med. 194: 59-63
    5. Dzionek, A. et al. (2002) Plasmacytoid dendritic cells: from specific surface markers to specific cellular functions. Hum. Immunol. 63: 1133-1148
    6. Ryan, E. J. et al. (2002) Dendritic cell-associated lectin-1: a novel dendritic cell-associated, C-type lectin-like molecule enhances T cell secretion of IL-4. J. Immunol. 169: 5638-5648
    7. Kerkmann, M. et al. (2003) Activation with CpG-A and CpG-B oligonucleotides reveals two distinct regulatory pathways of type I IFN synthesis in human plasmacytoid dendritic cells. J. Immunol. 170: 4465-4474
    8. Audige, A. et al. (2006)
      Anti-HIV state but not apoptosis depends on IFN signature in CD4
      +
      T cells.
      J. Immunol. 177: 6227-6237
Product options: 1
for 1×
10
9
total cells
CHF  821.00

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