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Human peripheral blood mononuclear cells (PBMCs) were spiked
with CD207-transfected 1881 cells to obtain a 5% initial frequency
cells were isolated using
CD207 (Langerin) MicroBeads, an MS Column and a MiniMACS™
Separator. Cells were stained with CD207 (Langerin)-PE (# 130-
098-355) and analyzed by flow cytometry using the MACSQuant®
Analyzer. Cell debris and dead cells were excluded from the analysis
based on scatter signals and propidium iodide fluorescence.
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