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dendritic cells (DCs) were isolated from spleen single-cell suspension using Anti-XCR1 MicroBead Kit (Spleen) with two MS Columns and a MiniMACS™ Separator. Cells were fluorescently stained with Anti-MHC Class II-Vio®
, Anti-XCR1-PE and CD8a-APC and analyzed using the MACSQuant®
Cell debris, dead cells, and autofluorescent cells were excluded from analysis based on scatters and propidium iodide fluorescence. Dot plots on the right show conventional DCs gated on CD11c+
MHC class II+
cells, stained for cross-presenting DC markers (CD8a and XCR1).
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