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PBMCs from a CMV+
donor were stimulated for 16 hours with CMV lysate. The responding cells were stained and isolated according to secretion of IL-4 using the IL-4 Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 24 IL-4 secreting CD4+
T cells were enriched per 106
T cells using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, two IL-4 secreting CD4+
T cells were detected per 106
T cells after enrichment. There seemed to be no difference between stimulated and unstimulated sample before enrichment.
*Please refer to the data sheet for detailed gating strategy and analysis.
** Percentage represents frequency among enriched cells.