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Pan B cells were isolated from spleen of a BALB/c mouse by using the Pan B Cell Isolation Kit II, an LS Column, and a MidiMACS™ Separator. The cells were fluorescently stained with CD19-APC (# 130-102-494) and CD45-FITC (# 130-102-491) and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
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