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cells were isolated from mouse spleen (A) or mouse bone marrow (B) using CD23 MicroBeads, an MS Column, and a MiniMACS™ Separator. Cells were fluorescently stained with CD23-PE (# 130-097-819) and CD45R (B220)-APC (# 130-091-843) and analyzed by flow cytometry using the MACSQuant®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.