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Untouched pan T cells were isolated from 30 mL of human EDTA-anticoagulated whole blood using the MACSxpress®
Whole Blood Pan T Cell Isolation Kit, a MACSmix™ Tube Rotator, and a MACSxpress Separator. The isolated cells were fluorescently stained with CD45‑VioBlue®
, CD3-FITC, CD3‑APC, CD2-PE, and CD56-PE and analyzed by flow cytometry using the MACSQuant®
Analyzer 10. Cell debris, non-leukocytes, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and propidium iodide fluorescence.
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