PepTivator AdV5 Penton, human

PepTivator AdV5 Penton, human

AdV5 Penton is a pool of lyophilized peptides, consisting mainly of 15-mer sequences with 11 amino acids overlap, covering the complete sequence of the AdV penton protein (UniProt ID: P12538).
In vitro
stimulation of antigen-specific T cells with PepTivator Peptide Pools causes the secretion of effector cytokines and the up-regulation of activation markers, which then allow the detection and isolation of antigen-specific T cells.

Background information

Adenoviruses (AdV) are non-enveloped, ubiquitous, lytic double-stranded DNA viruses. Up to now, 52 different human serotypes, divided into six species were described.
The penton protein is one of the structural proteins in the viral coat and is synthesized during late infection. Both AdV penton and hexon were described as immunodominant T cell targets.

Downstream applications

PepTivator AdV5 Penton – research grade has been specifically developed for efficient
in vitro
stimulation of AdV5 penton–specific T cells. Peptides of 15 amino acids in length and 11 amino acids overlap represent an optimized solution for stimulating both CD4
and CD8
T cells in various applications, including:
  • Detection and analysis of AdV5 penton–specific effector/memory T cells in PBMCs by MACS® Cytokine Secretion Assays, intracellular cytokine staining, or other technologies.
  • Isolation of viable AdV5 penton–specific CD4+ T cells with the CD154 MicroBead Kit, or of CD4+ and CD8+ T cells using the CD137 MicroBead Kit or MACS Cytokine Secretion Assay – Cell Enrichment and Detection Kits. Subsequently, cells can be expanded for generation of T cell lines.
  • Generation of AdV5 penton–specific effector/memory T cells from naive T cell populations.
  • Pulsing of antigen-presenting cells.
Product options: 1
stimulation of
CHF  196.00

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