Human IL-3

Human IL-3

IL-3 stands for interleukin 3. Human IL-3 is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Human IL-3 can be used for a variety of applications, including:
  • Induction of colony formation from hematopoietic progenitor cells in semi-solid medium in vitro , for example, CD34+ cells from umbilical cord blood2 .
  • In vitro differentiation studies, for example, of B lymphoid progenitors3 .
  • Cultivation of plasmacytoid dendritic cells4 .
  • In vitro expansion of hematopoietic stem cells.
  • Investigation of mast cell or basophil function, for example, basophil interaction with blood vessels5 .
  • Investigation of IL-3–mediated signaling pathways.

Background information

Interleukin 3 (IL-3) is a hematopoietic growth factor, which is produced mainly by activated T cells, but is also secreted by other cell types, including mast cells, eosinophils, and keratinocytes. The broad spectrum of biological activities of IL-3 includes the stimulation of the proliferation and differentiation of immature pluripotent hematopoietic stem cells and various lineage-committed progenitor cells, leading to the production of most of the major blood cell types. In addition, IL-3 also affects the functional activity of mature mast cells, basophils, eosinophils, and macrophages.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Biological activity

  • Proliferation of TF-1 cells (NIBSC 91/510)
  • premium grade: ≥ 2×
    10
    6
    IU/mg
    (typical activity: ≥ 2.3×
    10
    6
    IU/mg
    )
  • research grade: ≥ 1×
    10
    6
    IU/mg
  • Selected references

    1. Kitamura, T. et al. (1989) Establishment and characterization of a unique human cell line that proliferates dependently on GM-CSF, IL-3, or erythropoietin. J. Cell. Physiol. 140: 323-334
    2. Rossmanith, T. et al. (2001)
      Interleukin 3 improves the
      ex vivo
      expansion of primitive human cord blood progenitor cells and maintains the engraftment potential of SCID repopulating cells.
      Stem Cells 19: 313-320
    3. Crooks, G. M. et al. (2000)
      IL-3 increases production of B lymphoid progenitors from human CD34
      +
      CD38
      cells.
      J. Immunol. 165: 2382-2389
    4. Tas, S. W. et al. (2007) Noncanonical NF-kappaB signaling in dendritic cells is required for indoleamine 2,3-dioxygenase (IDO) induction and immune regulation. Blood 110: 1540-1549
    5. Lim, L. H. et al. (2006)
      Stimulation of human endothelium with IL-3 induces selective basophil accumulation
      in vitro.
      J. Immunol. 176: 5346-5353
    6. Jing, D. et al. (2010)
      Hematopoietic stem cells in co-culture with mesenchymal stromal cells--modeling the niche compartments
      in vitro
      .
      Haematologica 95(4): 542-550
    7. Narla, A. et al. (2011) Dexamethasone and lenalidomide have distinct functional effects on erythropoiesis. Blood 118(8): 2296-2304
    8. Steinleitner, K. et al. (2012) EVI1 and MDS1/EVI1 expression during primary human hematopoietic progenitor cell differentiation into various myeloid lineages. Anticancer Res. 32(11): 4883-4889
    9. Brault, J. et al. (2014)
      Optimized generation of functional neutrophils and macrophages from patient-specific induced pluripotent stem cells:
      ex vivo
      models of X
      0
      -linked, AR22
      0
      - and AR47
      0
      - chronic granulomatous diseases.
      Biores Open Access 3(6): 311-326
    10. Dighe N. et al. (2014) Long-term reproducible expression in human fetal liver hematopoietic stem cells with a UCOE-based lentiviral vector. PLoS One 9(8): e104805
    11. Laurenti, E. et al. (2015) CDK6 levels regulate quiescence exit in human hematopoietic stem cells. Cell Stem Cell 16(3): 302-313
  • Certificates

    Please follow this
    link
    to search for Certificates of Analysis (CoA) by lot number.
Product options: 7
E. coli
10 µg
CHF  185.00
E. coli
25 µg
CHF  285.00
E. coli
4×25 µg
CHF  720.00
E. coli
10 µg
CHF  225.00
E. coli
25 µg
CHF  365.00
E. coli
100 µg
CHF  720.00
E. coli
1000 µg
CHF  3'035.00

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