Clone:
REAL414
Type of antibody:
Releasable fluorochromes, Primary antibodies, Recombinant antibodies
Applications:
MICS, IF, IHC
Alternative names:
Ly-6G, Ly-6G.1

Specifications for Gr-1 Antibody, anti-mouse, REAdye_lease™

Overview

Clone REAL414 is an antibody fragment derived from the full Gr-1 antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAlease Complex to bind markers with high avidity.
Clone REAL414 recognizes the mouse Gr-1 antigen (granulocyte-differentiation antigen-1), also known as Ly-6G, a 21-25 kDa, GPI-anchored cell surface protein. Cross-reactivity of Anti-Gr-1 with Ly-6C was not detected on hematopoietic cells that express Ly-6C and are negative for Ly-6G.
Gr-1 is expressed on mature granulocytes in bone marrow and peripheral tissues. The Anti-Gr-1 antibody also stains monocytes transiently during their differentiation in bone marrow and at low levels plasmacytoid dendritic cells in lymphoid tissues.
For removal of REAdye_lease fluorochromes for optional relabeling with different fluorochrome-conjugated REAdye_lease antibodies use the REAlease Support Kit (130-120-675).

Alternative names

Ly-6G, Ly-6G.1

Detailed product information

Technical specifications

CloneREAL414
Clonalitymonoclonal
Isotype controlControl Antibody
Hostcell line
Type of antibodyReleasable fluorochromes, Primary antibodies, Recombinant antibodies
Speciesmouse
AntigenGr-1
Alternative names of antigenLy-6G, Ly-6G.1
Distribution of antigengranulocytes, monocytes, plasmacytoid dendritic cells, bone marrow

References for Gr-1 Antibody, anti-mouse, REAdye_lease™

Publications

  1. Fleming, T. J. et al. (1993) Selective expression of Ly-6G on myeloid lineage cells in mouse bone marrow. RB6-8C5 mAb to granulocyte-differentiation antigen (Gr-1) detects members of the Ly-6 family. J. Immunol. 151: 2399-2408
  2. Nagendra, S. and Schlueter, A. J. (2004) Absence of cross-reactivity between murine Ly-6C and Ly-6G. Cytometry A 58: 195-200