Clone:
15E8
Type of antibody:
Primary antibodies, Functional-grade antibodies
Isotype:
mouse IgG1
Applications:
FC, MICS, IF, IHC, ICC, FA
Alternative names:
Tp44, T44

Extended validation for CD28 Antibody, anti-human

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD28. Human peripheral blood mononuclear cells (PBMCs) were stained with CD28 antibodies and with a suitable counterstaining. As a control, CD28 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD28. Human peripheral blood mononuclear cells (PBMCs) were stained with CD28 antibodies and with a suitable counterstaining. As a control, CD28 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD28. Human peripheral blood mononuclear cells (PBMCs) were stained with CD28 antibodies and with a suitable counterstaining. As a control, CD28 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD28. Human peripheral blood mononuclear cells (PBMCs) were stained with CD28 antibodies and with a suitable counterstaining. As a control, CD28 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD28. Human peripheral blood mononuclear cells (PBMCs) were stained with CD28 antibodies and with a suitable counterstaining. As a control, CD28 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD28. Human peripheral blood mononuclear cells (PBMCs) were stained with CD28 antibodies and with a suitable counterstaining. As a control, CD28 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD28. Human peripheral blood mononuclear cells (PBMCs) were stained with CD28 antibodies and with a suitable counterstaining. As a control, CD28 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD28 (15E8). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD28 (15E8). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD28 (15E8). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD28 Antibody, anti-human

Overview

The CD28 antibody recognizes the human CD28 antigen, a type I transmembrane protein, which is highly expressed on CD3
+
thymocytes, most peripheral thymocytes and plasma cells. Binding of CD28 to its ligands CD80 or CD86 costimulates T cell effector function and T cell–dependent antibody production
in vitro
and
in vivo
. In the presence of antibodies directed against CD2 and CD3, CD28 antibodies stimulate T cell proliferation and cytokine production.

Alternative names

Tp44, T44

Detailed product information

Technical specifications

Clone15E8
Clonalitymonoclonal
Isotypemouse IgG1
Isotype controlIsotype Control Antibody, mouse IgG1
Hostmouse
Type of antibodyPrimary antibodies, Functional-grade antibodies
Specieshuman, non-human primate
Cross-reactivity
rhesus monkey (
Macaca mulatta
)
,
cynomolgus monkey (
Macaca fascicularis
)
AntigenCD28
Alternative names of antigenTp44, T44
Molecular mass of antigen [kDa]23
Distribution of antigenB cells, T cells, lymphocytes, plasma cells, thymocytes
Entrez Gene ID940
RRIDAB_2801822, AB_2752216, AB_2752213, AB_2889536, AB_2889535, AB_1036134, AB_1036117, AB_2660267, AB_2660268, AB_2660271, AB_871655, AB_2801826

Resources for CD28 Antibody, anti-human

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD28 Antibody, anti-human

Publications

  1. Kozbor D. J. et al. (1987) Tp44 molecules involved in antigen-independent T cell activation are expressed on human plasma cells. J. Immunol. 138(12): 4128-4132
  2. Bahlis, N. J. et al. (2007) CD28-mediated regulation of multiple myeloma cell proliferation and survival. Blood 109(11): 5002-5010
  3. Lécureuil, C. et al. (2007) Trapping and apoptosis of novel subsets of memory T lymphocytes expressing CCR6 in the spleen of HIV-infected patients. Blood 109: 3649-3657
  4. Krummel, M. F. and Allison, J. P. (1995) CD28 and CTLA-4 have opposing effects on the response of T cells to stimulation. J. Exp. Med. 182(2): 459-465
  5. Freeman, G. J. et al. (1993) Cloning of B7-2: a CTLA-4 counter-receptor that costimulates human T cell proliferation. Science 262(5135): 909-911
  6. Linsley, P. S. et al. (1993) The role of the CD28 receptor during T cell responses to antigen. Annu. Rev. Immunol. 11: 191-212
  7. June, C. H. et al. (1990) Role of the CD28 receptor in T-cell activation. Immunol. Today 11(6): 211-216

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