The CD146 MicroBead Kit was developed for the isolation of CD146
+
cells. The kit consists of CD146 MicroBeads and FcR Blocking Reagent. The CD146 MicroBeads recognize the human CD146 antigen, also known as MUC18, MCAM, Mel-CAM, and S-Endo-1.

Data and images for CD146 MicroBead Kit, human

Figures

Figure 1

CD146
+
cells were separated from lipoaspirate using the CD146 MicroBead Kit, an LS Column, and a MidiMACS™ Separator. Cells were stained with CD146-APC and CD34-FITC.
Unseparated fraction
CD146
cell fraction
View details

Figure 1

CD146
+
cells were separated from lipoaspirate using the CD146 MicroBead Kit, an LS Column, and a MidiMACS™ Separator. Cells were stained with CD146-APC and CD34-FITC.
View details

Figure 1

CD146
+
cells were separated from lipoaspirate using the CD146 MicroBead Kit, an LS Column, and a MidiMACS™ Separator. Cells were stained with CD146-APC and CD34-FITC.
CD146
+
cell fraction
View details

Figure 1

CD146
+
cells were separated from lipoaspirate using the CD146 MicroBead Kit, an LS Column, and a MidiMACS™ Separator. Cells were stained with CD146-APC and CD34-FITC.

Specifications for CD146 MicroBead Kit, human

Overview

The CD146 MicroBead Kit was developed for the isolation of CD146
+
cells. The kit consists of CD146 MicroBeads and FcR Blocking Reagent. The CD146 MicroBeads recognize the human CD146 antigen, also known as MUC18, MCAM, Mel-CAM, and S-Endo-1.

Detailed product information

Background information

CD146 is a transmembrane glycoprotein belonging to the immunoglobulin superfamily and contains five extracellular immunoglobulin (Ig)-like domains.
1,2
CD146 possesses a limited tissue expression distribution, including endothelial cells, smooth muscle cells, pericytes, follicular dendritic cells, melanoma cells, and a subpopulation of activated T lymphocytes.
1,3
CD146
+
cells isolated from umbilical cord
4
, lipoaspirate
5
, dental pulp, or endometrial tissue
6
, are described to contain cells with clonogenic fibroblastic colony (CFU-F) activity and multilineage differentiation potential, which are often called mesenchymal or marrow stromal cells (MSCs).
CD146 functions as a Ca
2+
-independent cell adhesion molecule, which is involved in heterophilic cell-cell interactions
7
, and may be involved in the extravasion and/or homing of activated T cells
3
. CD146 expression in melanoma is also directly associated with tumor growth and metastasis.
7,8

Applications

The CD146 MicroBead Kit can be used for:
  • isolation of human mesenchymal stromal cells (MSCs) from lipoaspirate and other tissue sources,
  • isolation of endothelial cells and pericytes from human endothelial tissue,
  • isolation of human umbilical vein endothelial cells (HUVECs) and human umbilical cord perivascular cells (HUCPVCs)4 from umbilical cord vein.

Columns

LS or XS Columns.

Resources for CD146 MicroBead Kit, human

References for CD146 MicroBead Kit, human

Publications

  1. Bardin, N. et al. (2001) Identification of CD146 as a component of the endothelial junction involved in the control of cell-cell cohesion. Blood 98: 3677-3684
  2. Yan, X. et al. (2003) A novel anti-CD146 monoclonal antibody, AA98, inhibits angiogenesis and tumor growth. Blood 102: 184-191
  3. Pickl, W. F. et al. (1997) MUC18/MCAM (CD146), an activation antigen of human T lymphocytes. J. Immunol. 158: 2107-2115
  4. Baksh, D. et al. (2007) Comparison of proliferative and multilineage differentiation potential of human mesenchymal stem cells derived from umbilical cord and bone marrow. Stem Cells 25: 1384-1392
  5. Zannettino, A. C. et al. (2008)
    Multipotential human adipose-derived stromal stem cells exhibit a perivascular phenotype
    in vitro
    and
    in vivo
    .
    J. Cell. Physiol. 214: 413-421
  6. Schwab and Gargett (2005) Reprod. Fertil. Dev. 17: 111-111
  7. Xie, S. et al. (1997) Expression of MCAM/MUC18 by human melanoma cell leads to increased tumor growth and metastasis. Cancer Res. 57: 2295-2303
  8. Satyamoorthy, K. et al. (2001) Mel-CAM-specific genetic suppressor elements inhibit melanoma growth and invasion through loss of gap junctional communication. Oncogene 20: 4676-4684

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