The Anti-Sca-1 MicroBead Kit (Vio
®
Bright FITC), mouse has been developed for the positive selection of hematopoietic stem and progenitor cells from mouse bone marrow. Sca-1
+
stem cells have been isolated from peripheral blood
1
, fetal
2
, and adult liver
3
, as well as heart
4
and prostate tissue
5
. The kit can be used either alone or in combination with the Lineage Cell Depletion Kit, mouse for enrichment of KSL cells as well as in combination with the CD105 MultiSort Kit (PE), mouse for the enrichment of

Data and images for
Anti-Sca-1 MicroBead Kit (Vio
®
Bright FITC)
, mouse

Figures

Figure 1

Sca-1
+
cells were isolated from bone marrow cells from BALB/c mice using the Anti-Sca-1 MicroBead Kit (VioBright™ FITC), two MS Columns, and a MiniMACS™ Separator. Cells were analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Before separation
Sca-1
+
cells
View details

Figure 1

Sca-1
+
cells were isolated from bone marrow cells from BALB/c mice using the Anti-Sca-1 MicroBead Kit (VioBright™ FITC), two MS Columns, and a MiniMACS™ Separator. Cells were analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
View details

Figure 1

Sca-1
+
cells were isolated from bone marrow cells from BALB/c mice using the Anti-Sca-1 MicroBead Kit (VioBright™ FITC), two MS Columns, and a MiniMACS™ Separator. Cells were analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for
Anti-Sca-1 MicroBead Kit (Vio
®
Bright FITC)
, mouse

Overview

The Anti-Sca-1 MicroBead Kit (Vio
®
Bright FITC), mouse has been developed for the positive selection of hematopoietic stem and progenitor cells from mouse bone marrow. Sca-1
+
stem cells have been isolated from peripheral blood
1
, fetal
2
, and adult liver
3
, as well as heart
4
and prostate tissue
5
. The kit can be used either alone or in combination with the Lineage Cell Depletion Kit, mouse for enrichment of KSL cells as well as in combination with the CD105 MultiSort Kit (PE), mouse for the enrichment of long-term repopulating hematopoietic stem cells (LT-HSCs).

Detailed product information

Background information

Stem cell antigen-1 (Sca-1) is one of the defining markers for KSL cells (c-
k
it
+S
ca-1
L
in
), the population that comprises the stem cell compartment in mouse bone marrow. Sca-1 expression in combination with CD105 can be further used to define long-term repopulating hematopoietic stem cells (LTR-HSCs).
6,7
The Sca-1 protein is encoded by one of the two Ly-6A/E alleles expressed in different mouse strains (Ly-6E.1: e.g. BALB/c, C3H, NZB; Ly-6A.2: e.g. C57BL/6, SJL, 129, AKR). The anti-Sca-1 monoclonal antibody included in the kit recognizes both, Ly-6E.1 and Ly-6A.2.

Downstream applications

Bone marrow-derived Sca-1
+
cells have been used for nonhematopoietic differentiation into hepatocytes
in vivo8
and neural cells
in vitro9
. ES cell-derived SCA-1
+
cells could be differentiated into endothelial cells with high purity and yield.
10
Furthermore, smooth muscle cells have been generated from ESCs via isolation of Sca-1
+
cells.
11

Columns

MS, LS, XS, or autoMACS
®
Columns.

Resources for
Anti-Sca-1 MicroBead Kit (Vio
®
Bright FITC)
, mouse

References for
Anti-Sca-1 MicroBead Kit (Vio
®
Bright FITC)
, mouse

Publications

  1. Yamada, M. et al. (2004) Bone marrow-derived progenitor cells are important for lung repair after lipopolysaccharide-induced lung injury. J. Immunol. 172: 1266-1272
  2. Cherqui, S. et al. (2006) Isolation and angiogenesis by endothelial progenitors in the fetal liver. Stem Cells 24: 44-54
  3. Petersen, B. E. et al. (2003) Mouse A6-positive hepatic oval cells also express several hematopoietic stem cell markers. Hepatology 37: 632-640
  4. Matsuura, K. et al. (2004) Adult cardiac Sca-1-positive cells differentiate into beating cardiomyocytes. J. Biol. Chem. 279: 11384-11391
  5. Burger, P. E. et al. (2005) Sca-1 expression identifies stem cells in the proximal region of prostatic ducts with high capacity to reconstitute prostatic tissue. Proc. Natl. Acad. Sci. U.S.A. 102: 7180-7185
  6. Chen, C. Z. et al. (2002) Identification of endoglin as a functional marker that defines long-term repopulating hematopoietic stem cells. Proc. Natl. Acad. Sci. U.S.A. 99: 15468-15473
  7. Chen, C. Z. et al. (2003) The endoglin(positive) sca-1(positive) rhodamine(low) phenotype defines a near-homogeneous population of long-term repopulating hematopoietic stem cells. Immunity 19: 525-533
  8. Lagasse, E. et al. (2000)
    Purified hematopoietic stem cells can differentiate into hepatocytes
    in vivo
    .
    Nat Med 6: 1229-1234
  9. Sanchez-Ramos, J. et al. (2000)
    Adult bone marrow stromal cells differentiate into neural cells
    in vitro
    .
    Exp. Neurol. 164(2): 247-256
  10. Xiao, Q. et al. (2006)
    Sca-1
    +
    progenitors derived from embryonic stem cells differentiate into endothelial cells capable of vascular repair after arterial injury.
    Arterioscler. Thromb. Vasc. Biol. 26: 2244-2251
  11. Zempetaki et al. (2007) Lacking cytokine production in ES cells and ES-cell-derived vascular cells stimulated by TNF-alpha is rescued by HDAC inhibitor trichostatin A. Am. J. Physiol., Cell Physiol. 293: C1126-1238

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Anti-Sca-1 MicroBead Kit (Vio
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Bright FITC)
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