Solutions for stimulation, enrichment, and analysis of rare virus-specific T cells 

Antigen-specific T cells play a pivotal role in fighting viral infections. Assessment of specificity, phenotype, and functional cytokine secretion of virus-activated T cells provides essential information on infection progression and the reactivity of the immune system.

However, assessment of ag-specific T cells is challenging as they constitute an extremely rare subset. Thus, a precise and sensitive analytic means is indispensable. 

Miltenyi Biotec offers several innovative products, protocols, and webinars to support you at every step of your workflow and accelerate your research
 

In vitro stimulation of  T cells and other immune cells

Enrichment of antigen-specific T cells

Flow analysis of antigen-specific T cells

  • Rapid Cytokine Inspector Kits are unique tools that combine surface marker and intracellular cytokine staining in an easy and fast way, while allowing precise analyses.
  • MACSplex Assays can be used if you are interested in analysis of multiple soluble cytokines in one sample.
  • MACS® Cytokine Secretion Assays are kits that enable you to detect and enrich viable cytokine-screening T cells at the level of one in a million, which is especially useful when viable antigen-specific T cells are needed for further down-stream applications.

 SARS-CoV-2 T Cell Analysis Kits

All-in-one Flow Kits for the analysis of SARS-CoV-2 reactive T cells (to be released soon). Each Kit will include a SARS-CoV-2 Peptivator of choice, the antibody panel and all needed controls and buffers.

Antibody panel in the Kit consists the following antibodies:

MarkerCloneFluorophore Order No. 
CD3REA613 APC 130-113-135
CD4REA623 Viobright-515 130-114-535
CD8REA734 VioGreen 130-110-684
IFN-g 45-15 PE 130-113-493
TNF-a cA2 PE-Vio770 130-120-492
CD14REA599 VioBlue 130-110-524
CD20REA780 VioBlue 130-111-531
CD154 (CD40L) 5C8 APC-Vio770 130-113-604
Live/dead Viobility Fixable Dye 405/452 130-109-816
Free channel*  PE-Vio615 n.a.

* Can be used for additional specificities of choice, e.g. IL-2, IL17, IL21, CD69, CD137, OX40, perforin.

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Webinar

MACS Academy: COVID-19 – Enrichment and analysis of rare antigen- and virus-specific T cells 

See how to beat the challenges that working with antigen- and virus-specific T cells in immunological research pose, specifically in SARS-CoV-2 and COVID-19 research.

Download  interactive PDF of the presentation

Related PDFs:

coming soon:

Fast and efficient detection of SARS-CoV-2 reactive T cells via ag-specific stimulation (application note)

For research requiring isolation of Pan T cells and T cell subsets
 

Untouched isolation of T cells and T cell subsets 

Direct isolation of T cells from blood and blood products

StraightFrom MicroBeads allow direct isolation of T cells from whole blood and blood products (Buffy Coat, LeukoPak® and LRSC) for e.g.  antigen-specific presentation in a Mixed Lymphocyte Reaction (MLR).

 

Related products:

Ficoll free solution to isolate PBMCs from whole blood

MACSPrep PBMC Isolation Kit, human

Check out more supporting materials on antigen-specific T cells