A complete workflow for reliable and efficient T helper cell (TH cell) differentiation, starting with cell isolation of naïve CD4+ T cells and in vitro activation and differentiation, through to comprehensive cell analysis.
Miltenyi Biotec provides gentle cell separation strategies, as well as reliable cell culture reagents such as T cell activation tools, T cell media and polarizing cytokines for the efficient differentiation of TH1, TH2 and TH17 cells.
Discover our T helper cell differentiation workflows
Application data by workflow step
T cell isolation
Isolation of naïve CD4+ T cells
Choose from a variety of MicroBeads and T cell Isolation kits for the separation of human and mouse naïve CD4+ T helper cells.
The Naïve T Cell Isolation Kits were developed for the isolation of untouched naïve T cells from human peripheral blood or mouse spleen single cell suspensions. Naïve T cells are ready for downstream applications, e.g., in studies on naive T helper cell activation and differentiation as well as and in functional studies of DCs.
Isolation of untouched naive CD4+ T helper cells from PBMCs using the Naive CD4+ T Cell Isolation Kit II, human. Cells were stained with CD4 and CD45RA antibodies and analyzed by flow cytometry using the MACSQuant® Analyzer.
Naïve CD4+ T cells were isolated from a single-cell suspension from mouse spleen using the Naive CD4+ T Cell Isolation Kit, mouse. Cells were fluorescently stained with CD45-VioGreen, CD4-VioBlue®, CD62L-PE, CD3ε-APC-Vio770™ , and CD44-FITC and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence and a gate was set on CD45+ CD3e+ CD4+ cells.
CD62L, CD45RA and CD45RO MicroBeads
Combine pre-enrichment with the CD4+ T cell Isolation Kits by positive selection or depletion with the respective MicroBeads to isolate even the rarest naïve CD4+ T cell populations.
T cell culture
T cell media
TexMACS™ Medium is an optimized, serum-free cell culture medium, developed for reliable T cell and Treg cell culture results. Chemically-defined and without animal-derived components, it allows for the highest reproducibility in human and mouse cell culture applications.
T cell activation
We offer a variety of polyclonal T cell stimulation tools for you to choose from. Find the options below:
We provide a broad selection of superior-quality cytokines, with high biological activity at a competitive price. Three different grades, research, premium, and MACS® GMP grade, provide flexibility in your assay setup.
Detection and enrichment of cytokine-producing T cells
Cytokine secretion assays are designed for highly sensitive detection and magnetic enrichment of viable cytokine-secreting cells. This unique technology enables detection of one antigen-specific T cell per million cells. Isolated cells can be immediately used for downstream applications, e.g., cell culture and flow cytometric analysis.
A range of kits are available for human and mouse cells secreting TNF-α, GMCSF, IFN-α, IFN-ƴ, or IL-2, -4, -5, -10, -12, -13, -17, -22.
Multiparameter flow analysis of T cells
As the final step in most workflows, flow analysis plays an important part in various T cell applications. Our flow reagents can help you ensure that the data you generate on your flow cytometer is reliable and consistent:
Build your own T cell flow panel with our Flow Panel Builder.