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Miltenyi Biotec-tested panel 20 (MBTP 20)
This application protocol describes the flow cytometric analysis of the activation status of NK cells isolated from PBMCs from whole blood of a healthy donor. Immunophenotyping of NK cells was performed on all PBMCs, directly after isolation via CD3 depletion and CD56 enrichment, and after 14 days of culture.
|Channel||Specificity||Clone||Purpose||Fluorochrome||Detection filter (nm) (laser)|
|V1||CD56||REA196||NK cells||Vio® Bright V423||450/50 (violet)|
|B1||CD335 (NKp46)||REA808||Activation marker||Vio Bright B515||525/50 (blue)|
|B2||CD336 (NKp44)||REA1163||Activation marker||PE||585/40 (blue)|
|B3||7-AAD Staining Solution||-||Viability||-||655–730 (blue)|
|B3||CD3||REA613||Cell type exclusion||PerCP-Vio 700||655–730 (blue)|
|CD14||REA599||Cell type exclusion|
|B4||CD337 (NKp30)||REA823||Activation marker||PE-Vio 770||750LP (blue)|
|R1||CD314 (NKG2D)||REA797||Activation marker||APC||655–730 (red)|
|R2||CD16||REA423||Maturation marker||APC-Vio 770||750LP (red)|
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