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In this application protocol, we describe an easy and convenient technique using MACSxpress® Technology for the efficient purification of untouched immune cells from human whole blood in 20 minutes. Up to 30 mL of whole blood can be processed in a single tube, enabling the fast isolation of a large number of NK cells for downstream applications. The procedure does not require density gradient centrifugation, which minimizes pipetting steps and aerosol formation for a high level of safety.
Isolate NK cells from up to 30 mL whole blood using the MACSxpress® Whole Blood NK Cell Isolation Kit, human. The kit enables isolation directly from blood in a single tube and in 20 minutes, without the need for density gradient centrifugation. Aggregated erythrocytes and platelets sediment, while magnetically labeled non-target cells are retained in the magnetic field of the MACSxpress Separator. The supernatant containing the target cells is then collected.
Follow the protocol of the kit data sheet.
Download data sheet
For NK cell expansion, PBMCs or isolated NK cells are cultured for up to 20 days in the presence of Anti-Biotin MACSiBead™ Particles loaded with CD2 and CD335 (NKp46) antibodies from the NK Cell Activation/Expansion Kit, human. Follow the protocol of the kit data sheet.
Download data sheet
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