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BALB/c mice were immunized and the spleen cells restimulated with KLH (keyhole limpet hemocyanin). The responding cells were stained according to secretion of IL-4 using the Mouse IL-4 Secretion Assay - Detection Kit.
In the stimulated sample, 0.360% of all CD4+
T cells secreted IL-4. In the unstimulated control sample, only 0.027% of all CD4+
T cells secreted IL-4.
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