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BALB/c mice were immunized i.p. with KLH (keyhole limpet hemocyanin) in incomplete Freund's adjuvant and pertussis toxin. On day 21 after immunization, mouse spleen cells were restimulated in vitro with KLH for 15 hours. The responding cells were stained for coexpression of cytokines using the Mouse IFN-γ Secretion Assay - Detection Kit (PE) and Mouse IL-2 Secretion Assay - Detection Kit (APC). The plots show IFN-γ- and IL-2-secreting cells gated on viable CD4+
T cells for the stimulated sample as well as for the unstimulated control.
* Percentage represents frequency among CD4+ T cells.
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