REAlease® CD115 Complexes, mouse

REAlease
®
CD115 Complexes
, mouse

Clone: REAL272 | Dilution: 1:50
Clone REAL272 is an antibody fragment derived from the full CD115 antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAlease Complex to bind markers with high avidity.
Clone REAL272 recognizes the protein tyrosine kinase transmembrane receptor for macrophage colony-stimulating factor (M-CSF), also known as CSF-1 and interleukin 34 (IL-34). CD115 is a single-pass type I membrane protein. Receptor activation induces homo-dimerization, which leads to phosphorylation and ubiquitinylation of intracellular residues. CD115 is expressed on mouse monocytes, macrophages, and osteoclasts, as well as on common dendritic cell precursors and macrophage/dendritic cell precursors.
The REAlease Kits consist of the respective fluorochrome-conjugated REAlease Complexes and the REAlease Support Kit for removal of the REAlease Complexes and optional relabeling with different fluorochrome-conjugated REAlease Complexes.

Alternative names

Csfmr, Fim-2, FMS, M-CSFR, CSF-1R, c-fms

Technical specifications

  • Antigen: CD115
  • Clone: REAL272
  • Isotype control: REAL Control
  • Alternative names of antigen: Csfmr, Fim-2, FMS, M-CSFR, CSF-1R, c-fms
  • Distribution of antigen: macrophages, monocytes, osteoclasts, dendritic cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: APC, PE
  • Selected references

    1. Sudo, T. et al. (1995) Functional hierarchy of c-kit and c-fms in intramarrow production of CFU-M. Oncogene 11: 2469-2476
    2. Murayama, T. et al. (1999) Intraperitoneal administration of anti-c-fms monoclonal antibody prevents initial events of atherogenesis but does not reduce the size of advanced lesions in apolipoprotein E-deficient mice. Circulation 99: 1740-1746
    3. Sunderkötter, C. et al. (2004) Subpopulations of mouse blood monocytes differ in maturation stage and inflammatory response. J. Immunol. 172: 4410-4417
    4. Auffray, C. et al. (2009)
      CX
      3
      CR1
      +
      CD115
      +
      CD135
      +
      common macrophage/DC precursors and the role of CX
      3
      CR1 in their response to inflammation.
      J. Exp. Med. 206(3): 595-606
    5. Breslin W. L. et al. (2013) Mouse blood monocytes: Standardizing their identification and analysis using CD115. J. Immunol. Methods 390(1-2): 1-8
Product options: 3
30 µg in 200 µL (1)
-
30 µg in 200 µL (1)
-
for 100
tests
-

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