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Human peripheral blood mononuclear cells (PBMCs) were stained with CD56 antibodies or with the corresponding REA Control (S) antibodies (left images) as well as with CD3 antibodies. Cells were then analyzed by flow cytometry using the MACSQuant®
Analyzer. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.
|Other clones||Overlap in epitope recognition with REA196|
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