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C57BL/6 splenocytes were either stained with CD55 (DAF) antibodies conjugated to (A) FITC, (B) PE, (C) APC, (D) PE-Vio770, (E) APC-Vio770, and (F) Biotin and analyzed by flow cytometry using the MACSQuant®
Analyzer. The specificity of the conjugated antibodies is confirmed by blocking the binding to ligand, using pure unconjugated antibodies (control: left images). CD45.2+
cells were pre-gated for the analysis. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of CD55 (DAF)-PE-Vio770 and CD55 (DAF)-APC-Vio770.
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