CD44 antibodies, mouse

CD44 antibodies, mouse

Clone: REA664 | Dilution: 1:10
Clone REA664 recognizes the mouse CD44 antigen, which is also known as Pgp-1, HCELL, HUTCH-I, HCAM, HERMES, or Ly-24. CD44 is a 80–95 kDa membrane glycoprotein and expressed by a variety of hematopoetic and non-hematopoetic cells. Different isoforms are generated by alternative splicing. Clone REA664 reacts with all isoforms and both allelic forms of CD44. CD44 functions as a receptor for hyaluronic acid (HA) and mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). The interaction between galectin-9 and CD44 increases the stability and function of adaptive regulatory T cells. CD44 expression is upregulated on naive T cells upon activation and high CD44 expression is maintained on memory T cells. Therefore, CD44 is often used to discriminate CD44
high
antigen–experienced T cells from CD44
low
(CD62L
+
) naive T cells.
Additional information: Clone REA664 displays negligible binding to Fc receptors.

Alternative names

CD44s, EMCR III, H-CA, Pgp-1, HERMES, Ly-24, HUTCH-I, Hermes antigen

Technical specifications

  • Antigen: CD44
  • Clone: REA664
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control antibodies
  • Alternative names of antigen: CD44s, EMCR III, H-CA, Pgp-1, HERMES, Ly-24, HUTCH-I, Hermes antigen
  • Entrez Gene ID: 12505
  • Molecular mass of antigen [kDa]: 83
  • Cross-reactivity:
    rhesus monkey (
    Macaca mulatta
    )
    ,
    cynomolgus monkey (
    Macaca fascicularis
    )
    ,
    chimpanzee (
    Pan troglodytes
    )
    , baboon, human, pig, cat, dog, cow, horse
  • Distribution of antigen: bone marrow, cancer cells, CNS cells, endothelial cells, epithelial cells, kidney, leukocytes, lymphocytes, mesenchymal stem cells, myeloid cells, plasma cells, ES and iPS cells, red blood cells, skeletal muscle, skin, T cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE-Vio770, Biotin, PE
  • Selected references

    1. He, Q. et al. (1992) Molecular isoforms of murine CD44 and evidence that the membrane proximal domain is not critical for hyaluronate recognition. J. Cell Biol. 119(6): 1711-1719
    2. Ponta, H. et al. (2003) CD44: From adhesion molecules to signalling regulators. Nat. Rev. Mol. Cell Biol. 4(1): 33-45
    3. Wu, C. et al. (2014) Galectin-9-CD44 interaction enhances stability and function of adaptive regulatory T cells. Immunity 41(2): 270-282
Product options: 3
30 µg in 1 mL (1)
-
9 µg in 300 µL (1)
-
30 µg in 1 mL (1)
-