CD32 antibodies, rat

CD32 antibodies, rat

Clone: REA256 | Dilution: 1:10
Clone REA256 recognizes the CD32 antigen, a 40 kDa polymorphic transmembrane glycoprotein also known as low affinity immunoglobulin gamma Fc region receptor II (FcγRII or FCRII). CD32 is a surface receptor protein and part of a large population of B cell co-receptors, which act to modulate signaling. Rat CD32 is expressed by B lymphocytes, myeloid cells, some lymphocytes in the thymic medulla, and cells which appear homologous to plasmacytoid dendritic cells. It mediates phagocytosis and oxidative burst in granulocytes, as well as platelet aggregation and immunomodulation. The extracellular domain of CD32 binds to aggregated IgG and immune complexes and down-regulates antibody production in the presence of IgG. This feedback loops essentially lowers the production of IgG produced by B cells when there is a surplus in the body.
Additional information: Clone REA256 displays negligible binding to Fc receptors.

Alternative names

FCGR2B, FcRII, FcgammaRIIB2, Fcgr2

Technical specifications

  • Antigen: CD32
  • Clone: REA256
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control antibodies
  • Alternative names of antigen: FCGR2B, FcRII, FcgammaRIIB2, Fcgr2
  • Entrez Gene ID: 289211
  • Molecular mass of antigen [kDa]: 28
  • Distribution of antigen: B cells, NK cells, granulocytes, macrophages, platelets, monocytes
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE, APC, PE-Vio770, APC-Vio770, Biotin
  • Selected references

    1. Hubert, F. X. et al. (2004)
      Rat plasmacytoid dendritic cells are an abundant subset of MHC Class II
      +
      CD4
      +
      CD11b
      OX62
      and type I IFN-producing cells that exhibit selective expression of Toll-like receptors 7 and 9 and strong responsiveness to CpG.
      J. Immunol. 172(12): 7485-7494
    2. Lynch, R. G. et al. (2000) Regulatory roles for FcgammaRIII (CD16) and FcgammaRII (CD32) in the development of T- and B-lineage lymphoid cells. J. Leukoc. Biol. 67: 279-284
Product options: 10
130-103-388

CD32-PE, rat

9 µg in 300 µL (1)
-
130-102-749

CD32-PE, rat

30 µg in 1 mL (1)
-
130-103-389

CD32-APC, rat

9 µg in 300 µL (1)
-
130-102-750

CD32-APC, rat

30 µg in 1 mL (1)
-
9 µg in 300 µL (1)
-
30 µg in 1 mL (1)
-
9 µg in 300 µL (1)
-
30 µg in 1 mL (1)
-
130-102-131

CD32-Biotin, rat

9 µg in 300 µL (1)
-
130-101-985

CD32-Biotin, rat

30 µg in 1 mL (1)
-

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