CD304 (BDCA-4) antibodies, human

CD304 (BDCA-4) antibodies, human

Clone: AD5-17F6 | Dilution: 1:50
Clone AD5-17F6 recognizes the human CD304 (BDCA-4) antigen. Like CD303 (BDCA-2), the CD304 (BDCA-4) antigen is specifically expressed on human plasmacytoid dendritic cells in blood and bone marrow. Unlike CD303 (BDCA-2), expression of CD304 (BDCA-4) is up-regulated on plasmacytoid dendritic cells and induced on myeloid cells upon culturing. In inflamed tonsils, CD304 (BDCA-4) expression, apart from plasmacytoid dendritic cells, is also detected primarily on follicular B helper memory T cells. CD304 (BDCA-4) is identical to neuropilin-1 (NP-1), a neuronal receptor, which is known to be expressed on numerous nonhematopoietic cell types, including neurons, endothelial, and tumor cells. CD1a
+
dendritic cells generated
ex vivo
from monocytes or hematopoietic precursor cells are CD304 (BDCA-4)
+
but CD303 (BDCA-2)
.

Alternative names

NRP1, BDCA-4, NP1, Nrp, VEGF165R

Technical specifications

  • Antigen: CD304 (BDCA-4)
  • Clone: AD5-17F6
  • Isotype: mouse IgG1
  • Isotype control: Mouse IgG1 – isotype control antibodies
  • Alternative names of antigen: NRP1, BDCA-4, NP1, Nrp, VEGF165R
  • Entrez Gene ID: 8829
  • Molecular mass of antigen [kDa]: 101
  • Distribution of antigen: CNS cells, dendritic cells, endothelial cells, heart, kidney, liver, neurons, placenta, T cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: VioBright FITC, PE, APC, PE-Vio770, Biotin
  • Selected references

    1. Mittag, D. et al. (2011) Human dendritic cell subsets from spleen and blood are similar in phenotype and function but modified by donor health status. J. Immunol. 186(11): 6207-6217
    2. Dzionek, A. et al. (2000) BDCA-2, BDCA-3, BDCA-4: Three markers for distinct subsets of dendritic cells in human peripheral blood. J. Immunol. 165: 6037-6046
    3. Jongbloed, S. L. et al. (2010)
      Human CD141
      +
      (BDCA-3)
      +
      dendritic cells (DCs) represent a unique myeloid DC subset that cross-presents necrotic cell antigens.
      J. Exp. Med. 207(6): 1247-1260
    4. Dzionek, A. et al. (2001) BDCA-2, a novel plasmacytoid dendritic cell–specific type II C-type lectin, mediates antigen capture and is a potent inhibitor of interferon α/β induction. J. Exp. Med. 194: 1823-1834
    5. Moschella, F. et al. (2013) Cyclophosphamide induces a type I interferon-associated sterile inflammatory response signature in cancer patients' blood cells: implications for cancer chemoimmunotherapy. Clin. Cancer Res. 19(15): 4249-4261
    6. Dzionek, A. et al. (2002) Plasmacytoid dendritic cells: from specific surface markers to specific cellular functions. Hum. Immunol. 36: 1133-1148
    7. Grabbe, S. et al. (2000) Dendritic cells: multi-lineal and multi-functional. Immunol. Today 21: 431-433
    8. Jardine, L. et al. (2013) Rapid detection of dendritic cell and monocyte disorders using CD4 as a lineage marker of the human peripheral blood antigen-presenting cell compartment. Front Immunol 4: 495
  • Customer reports and application notes

Product options: 10
for 30
tests
(1)
-
for 100
tests
(1)
-
for 30
tests
(1)
-
for 100
tests
(1)
-
for 30
tests
(1)
-
for 100
tests
(1)
-
for 30
tests
(1)
-
for 100
tests
(1)
-
for 30
tests
(1)
-
for 100
tests
(1)
-

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