Clone REA110 recognizes CD159a, an inhibitory natural killer (NK) cell receptor. CD159a forms heterodimer with CD94 and contains a C-type lectin ectodomain. Inhibitory signal is transmitted by the heterodimer via immunoreceptor tyrosine-based inhibitory motifs (ITIMs) and upon ligand engagement, ITIMs are phosphorylated and transmit signal through docking of various tyrosine phosphatases. CD159a/CD94 binds non-classical MHC class I protein, HLA-E. Expression of CD159a is found mainly on NK cells and on subsets of CD8
+
cells.
Additional information: Clone REA110 displays negligible binding to Fc receptors.

Alternative names

KLRC1, CD159a, NKG2, NKG2A

Technical specifications

  • Antigen: CD159a (NKG2A)
  • Clone: REA110
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Alternative names of antigen: KLRC1, CD159a, NKG2, NKG2A
  • Entrez Gene ID: 3821
  • Molecular mass of antigen [kDa]: 26
  • Cross-reactivity:
    chimpanzee (
    Pan troglodytes
    )
    ,
    olive baboon (
    Papio anubis
    )
    ,
    rhesus monkey (
    Macaca mulatta
    )
    ,
    cynomolgus monkey (
    Macaca fascicularis
    )
  • Distribution of antigen: NK cells, T cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, VioBright FITC, PE, APC, PE-Vio770, Biotin, PE-Vio615
  • Selected references

    1. Rueda, C. et al. (2016)
      Lipopolysaccharide-induced chorioamnionitis promotes IL-1-dependent inflammatory FOXP3
      +
      CD4
      +
      T cells in the fetal rhesus macaque.
      J. Immunol. 196(9): 3706-3715
    2. Boyington, J. C. et al. (1999) Structure of CD94 reveals a novel C-type lectin fold: implications for the NK cell-associated CD94/NKG2 receptors. Immunity 10(1): 75-82
    3. Sullivan, L. C. et al. (2007) The heterodimeric assembly of the CD94-NKG2 receptor family and implications for human leukocyte antigen-E recognition. Immunity 27(6): 900-911
    4. Reeves R. K. et al. (2015) Antigen-specific NK cell memory in rhesus macaques. Nat. Immunol. 16: 927-932
    5. Veluchamy, J. P. et al. (2017) High-efficiency lysis of cervical cancer by allogeneic NK cells derived from umbilical cord progenitors is independent of HLA status. Cancer Immunol. Immunother. 66: 51-61
    6. Wolpert, F. et al. (2015) Interferon-β modulates the innate immune response against glioblastoma initiating cells. PLoS One 10(10): e0139603
    7. Borrego, F. et al. (2006) The CD94/NKG2 family of receptors: from molecules and cells to clinical relevance. Immunol. Res. 35(3): 263-278
    8. Croci, S. et al. (2018) Higher frequencies of lymphocytes expressing the natural killer group 2D receptor in patients with Behçet disease. Front Immunol 9: 2157
    9. Jacomet, F. et al. (2017)
      The hypothesis of the human iNKT/innate CD8
      +
      T-cell axis applied to cancer: evidence for a deficiency in chronic myeloid leukemia.
      Front Immunol 7: 688
    10. Mahaweni, N. et al. (2018)
      NKG2A expression is not
      per se
      detrimental for the anti-multiple myeloma activity of activated natural killer cells in an
      in vitro
      system mimicking the tumor microenvironment.
      Front Immunol 9: 1415
    11. Rechtien, A. et al. (2017) Systems vaccinology identifies an early innate immune signature as a correlate of antibody responses to the Ebola vaccine rVSV-ZEBOV. Cell Rep 20(9): 2251-2261
  • Customer reports and application notes

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