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As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
Splenocytes from a KLH-immunized mouse were restimulated in vitro with or without KLH, CD28, and CD40 antibodies for six hours. Cells were harvested, stained with CD4-FITC and CD154-PE, or CD154-Biotin and Anti-Biotin-PE, and analyzed by flow cytometry. A lymphocyte gate based on forward and side scatter properties was activated. Dead cells and B cells were excluded according to PI and CD45R/B220-PerCP™ staining.
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