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Human umbilical vein endothelial cells (HUVEC), either left unstimulated (left peak) or stimulated with 10 ng/mL tumor necrosis factor α (TNF- α) for 20 hours, were stained with CD106 (VCAM-1) antibodies and analyzed by flow cytometry using the MACSQuant®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
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