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MOLT-4 cells were either left unstimulated (left image) or stimulated with 400 ng/mL phorbol myristate acetate (PMA) for 24 hours. Cells were then fixed and permeabilized using the Cell Signaling Buffer Set A followed by intracellular staining with Anti-Under-phospho Rb antibodies. Flow cytometry was performed using the MACSQuant®
Analyzer. Cell debris were excluded from the analysis based on scatter signals.
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