Clone REA401 recognizes the human, mouse, and rat ventricular isoform of the myosin light chain 2 (MLC2v) antigen. Myosin is a hexamer composed of two myosin heavy chains and four myosin light chains. In the heart two different forms of the myosin light chain are expressed, the atrial form (MLC2a) and the ventricular form (MLC2v). MLC2v is restricted to the ventricular segment of the heart tube at embryonic day 8 in rodents, suggesting that its ventricular specification occurs relatively early during mammalian cardiogenesis. In contrast, the atrial isoform of the myosin light chain 2 (MLC2a) is expressed in the presumptive ventricle prior to MLC2v, and its ventricular expression is subsequently down-regulated. In the fetal stage, MLC2a is primarily found in the atria while MLC2v is essentially restricted to the ventricles, albeit low levels of both MLC2a and MLC2v persist in the inflow tract, the atrioventricular canal and the outflow tract of the heart. MLC2v may as well be suitable to identify mouse and human pluripotent stem cell-derived cardiomyocyte subtypes.
Additional information: Clone REA401 displays negligible binding to Fc receptors.