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Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-iNKT antibodies as well as with CD3 antibodies and analyzed by flow cytometry using the MACSQuant®
Analyzer. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.
Rhesus monkey PBMCs (A) or cynomolgus monkey PBMCs (B) were stained with Anti-iNKT-APC as well as with CD3-PE and CD45-FITC and analyzed using the MACSQuant®
Analyzer. Cells were gated on CD45+
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