Find the products and resources you are looking for!
Miltenyi Biotec distribution:
As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
Human peripheral blood mononuclear cells (PBMCs) were incubated with or without (left images) LPS for six hours. After two hours, brefeldin A was added. Cells were harvested, then stained with CD14 antibodies, fixed, permeabilized, and intracellularly stained with Anti-IL-6 antibodies. The FcR Blocking Reagent has been used to avoid Fc receptor–mediated antibody labeling. Cells were analyzed by flow cytometry using the MACSQuant®
Analyzer. Gating was performed on monocytes according to scatter properties of the cells. Autofluorescent cell debris was excluded from the analysis in a fluorescence 4 (FL-4/B3) versus fluorescence 3 (FL-3/B2) dot plot.
Copyright © 2019 Miltenyi Biotec and/or its affiliates. All rights reserved.