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mouse brain tissue postnatal day 5 was dissociated using the Neural Tissue Dissociation Kit (T) and the gentleMACS™ Dissociator. Brain cells were intracellularly stained with Anti-GFAP antibodies or with the corresponding REA Control (I) antibodies (left images) as well as with Anti-GLAST (ACSA-1) antibodies. Flow cytometry was performed using the MACSQuant®
Analyzer. Cell debris were excluded from the analysis based on scatter signals.
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