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Human whole blood cells were stained with Anti-fMLP receptorantibodies, as well as with CD14 antibodies followed by erythrocyte lysis. Cells were then analyzed by flow cytometry using the MACSQuant®
Analyzer. The specificity of the conjugated antibodies was confirmed by blocking the binding t othe ligand, using pure unconjugated antibodies (left). Cell debri sand dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
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