Anti-CLIP antibodies, human

Anti-CLIP antibodies, human

Clone: REA296 | Dilution: 1:11
Clone REA296 recognizes MHC class II-associated invariant chain (Ii)-derived peptide (CLIP) complexes. MHC class II αβ heterodimers associate early during biosynthesis with a type II membrane protein, the invariant chain (Ii). The invariant chain serves as a chaperone for MHC II molecules and mediates trafficking to the endosomal pathway. In the endosomal pathway Ii is sequentially degraded, leaving a residual CLIP in the peptide-binding groove of MHC II. In presence of antigen peptide fragments, HLA-DM then binds to the MHC II molecule, releasing CLIP and allowing peptides to bind. REA296 detects HLA class II-positive cells which have impaired HLA-DM activity, and tumor cells that have escaped immuno-surveillance by CD4-positive T cells.
Additional information: Clone REA296 displays negligible binding to Fc receptors.

Alternative names

Class II-associated invariant chain peptide, DHLAG, HLADG, II, Ia-GAMMA, CD74

Technical specifications

  • Antigen: CLIP
  • Clone: REA296
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Alternative names of antigen: Class II-associated invariant chain peptide, DHLAG, HLADG, II, Ia-GAMMA, CD74
  • Entrez Gene ID: 972
  • Molecular mass of antigen [kDa]: 34
  • Distribution of antigen: B cells, dendritic cells, macrophages, monocytes
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, PE, APC, PE-Vio770, Biotin
  • Selected references

    1. van Bergen, J. et al. (1997) Efficient loading of HLA-DR with a T helper epitope by genetic exchange of CLIP. Proc. Natl. Acad. Sci. U.S.A. 94(14): 7499-7502
    2. Wälchli, S. et al. (2014) Invariant chain as a vehicle to load antigenic peptides on human MHC class I for cytotoxic T-cell activation. Eur. J. Immunol. 44(3): 774-784
    3. Bakke, O. et al. (1990) MHC class II-associated invariant chain contains a sorting signal for endosomal compartments. Cell 63(4): 707-716
Product options: 10
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for 100
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for 30
tests
(1)
-
for 100
tests
(1)
-
for 30
tests
(1)
-
for 100
tests
(1)
-
for 30
tests
(1)
-
for 100
tests
(1)
-

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