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Separation of a buffy coat sample using the StraightFrom®
Buffy Coat CD4 MicroBead Kit and the MultiMACS™ Cell24 Separator Plus with the Single-Column Adapter and Whole Blood Columns. Cells were fluorescently stained with CD4-PE, CD14-APC, as well as CD45-VioBlue®
and analyzed by flow cytometry using the MACSQuant®
Analyzer. Cells were triggered via CD45-VioBlue, cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
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