Anti-MHC Class II MicroBeads, mouse

Anti-MHC Class II MicroBeads, mouse

Mouse Anti-MHC Class II MicroBeads are used for the positive selection or depletion of MHC class II
+
cells from lymphoid and non-lymphoid tissue or from blood.

Background information

MHC class II is expressed on B cells, macrophages, monocytes, dendritic cells, thymic epithelial cells, and on subsets of hematopoietic progenitor cells. The MicroBeads bind to MHC class II haplotypes I-A
b
, I-A
d
, I-A
q
, I-E
d
, and I-E
k
, which are expressed by the most common inbred mouse strains, e.g. C57BL/6, BALB/c, or 129/SvEv. MHC II haplotypes I-A
f
, I-A
k
, and I-A
s
are not recognized.

Downstream applications

Isolated MHC class II
+
cells are suitable for studies on antigen presentation such as in T cell stimulation assays. Anti-MHC Class II MicroBeads were used for positive selection of Langerhans cells from epidermis
1
, for the isolation of untouched T cells from lymph nodes by depletion of MHC class II
+
cells
2
, or in combination with other MicroBeads for isolation of untouched T cells from spleen
3,4
.

Columns

For positive selection: MS, LS, XS, or autoMACS
®
Columns. For depletion: LD, D, or autoMACS Columns.
  • Selected references

    1. Sato, T. et al. (2002) Consequences of OX40-OX40 ligand interactions in langerhans cell function: enhanced contact hypersensitivity responses in OX40L-transgenic mice. Eur. J. Immunol. 32: 3326-3335
    2. Fruman, D. A. et al. (1999) Impaired B cell development and proliferation in absence of phosphoinositide 3-kinase p85alpha. Science 283: 393-397
    3. Lerner, C. G. et al. (2000) Distinct requirements for C-C chemokine and IL-2 production by naive, previously activated, and anergic T cells. J. Immunol. 164: 3996-4002
    4. Foulds, K. E. et al. (2002) Cutting edge: CD4 and CD8 T cells are intrinsically different in their proliferative responses. J. Immunol. 168: 1528-1532
Product options: 1
for 2×
10
9
total cells
-

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