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Positive selection of CX3CR1+
cells from PBMCs using the Anti-CX3CR1 MicroBead Kit, an LS Column and a MidiMACS™ Separator. Cells were fluorescently stained with Anti-CX3CR1-PE and analyzed by flow cytometry using the MASCQuant®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
PBMCs before separation
PBMCs depleted of CX3CR1+
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