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Enrichment of dead (apoptotic/necrotic) cells using the Annexin V MicroBead Kit. Murine thymocytes were treated with CD95 (anti-Fas) monoclonal antibody for 3 hours at 37 °C. PS-exposing cells were enriched by labeling with Annexin V MicroBeads followed by separation on an MS Column in the magnetic field of a MiniMACS™ Separator. Cell fractions were stained with Annexin V-FITC.
Murine thymocytes after treatment with CD95 (anti-Fas) antibody
Positive fraction enriched for PS-exposing cells
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